Human Induced Pluripotent Stem Cell (HiPSC) Workshop
Date and Location:
August 13-15, 2012
170 College St., Mining Building, Room 325
Induced pluripotent stem cell (iPSC) technology has been pushed steadily by technical advancements and biological analyses, leading this still relatively new discovery to the forefront of stem cell research. Broad-range applications of the technique include disease modeling, drug screening and the promise for patient-matched cellular replacement therapies. Gaining expertise in reprogramming has relevance across a spectrum of research disciplines. By the end of this workshop, participants should understand and be able to apply the processes involved in the production and maintenance of iPSCs, as well as gain a strong foundation in standardized validation and characterization methods, ensuring the quality of the stem cell lines produced. In addition, participants will begin building a national and international network of colleagues whose interests are in iPSC technology.
Workshop Key Learning Objectives:
- Theory of iPSC production and underlying technical methodologies applied to achieve reprogramming.
- Hands-on basic cell culture for human ESCs, iPSCs and primary fibroblast lines, including media preparation, colony picking and expansion, cryopreservation and revival.
- Hands-on induction of fibroblasts and blood cells using various transgene delivery methods.
- Hands-on identification of emergent iPSC colonies and their isolation.
- Characterization of established iPSCs via immunostaining and quantitative PCR, as well as high-throughput methods using flow cytometry.
- Differentiation of iPSCs along desired embryonic lineages.
Registration and Application, Fees and Deadlines:
Register by July 13, 2012 - Space is Limited!
Some financial support is available to eligible trainees and PIs through the Stem Cell Network (SCN). The deadline to apply is Monday, June 25th. Financial support information.
All others, please register below.
Industry - $1,500 + HST
Academic/trainee - $1,000 + HST
For sponsorship or questions, contact us at: firstname.lastname@example.org
Workshop Leader and Hosts:
Human Induced Pluripotent Stem Cell (HiPSC) Workshop is led by Dr. Kamal Garcha, Director of the Cell Reprogramming & Engineering Platform of the Centre for Commercialization of Regenerative Medicine (CCRM).
Thank you to our sponsors and hosts: Centre for Commercialization of Regenerative Medicine, Stem Cell Network and University of Toronto.