Date and Location:
December 11-13, 2013
University of Toronto, St. George Campus
Induced pluripotent stem cell (iPSC) technology has been pushed steadily by technical advancements and biological analysis, leading this still relatively new discovery to the forefront of stem cell research. Broad-range applications of the technique include disease modeling, drug screening and the promise for patient-matched cellular replacement therapies. Gaining expertise in reprogramming has relevance across a spectrum of research disciplines. By the end of this workshop, participants should understand and be able to apply the processes involved in the production and maintenance of iPSCs, as well as gain a strong foundation in standardized validation and characterization methods, ensuring the quality of the stem cell lines produced. In addition, participants will begin building a national and international network of colleagues whose interests are in iPSC technology.
Workshop Key Learning Objectives:
- Theory of iPSC production and underlying technical methodologies applied to achieve reprogramming.
- Hands-on basic cell culture for human ESCs, iPSCs and primary fibroblast lines, including media preparation, colony picking and expansion, cryopreservation and revival.
- Hands-on induction of fibroblasts and blood cells using various transgene delivery methods.
- Hands-on identification of emergent iPSC colonies and their isolation.
- Characterization of established iPSCs via immunostaining and quantitative PCR, as well as high-throughput methods using flow cytometry.
- Differentiation of iPSCs along desired embryonic lineages.
Agenda: (full pdf)
Registration and Application, Fees and Deadlines:
Register by November 1, 2013 - Space is Limited!
Industry - $2,000
Industry Consortium - $1,500
Academic - $1,000
Financial support is available to eligible participants through the Stem Cell Network (SCN). Please click here for details.
For sponsorship or questions, contact us at: firstname.lastname@example.org
Workshop Leader and Hosts:
Human Induced Pluripotent Stem Cell (HiPSC) Workshop is led by cell reprogramming experts at CCRM and invited speakers.
Thank you to the Stem Cell Network for sponsorship and to our host, the University of Toronto.